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pbi121 vector  (TaKaRa)


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    Structured Review

    TaKaRa pbi121 vector
    Pbi121 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14677 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbi121+vector/BamH+I/pm40511549-158-1-7
    Average 99 stars, based on 14677 article reviews
    pbi121 vector - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Interaction of EXA1 and eIF4E Family Members Facilitates Potexvirus Infection in Arabidopsis thaliana.
    Article Snippet: Plant viruses depend on a number of host factors for successful infection.. Deficiency of critical host factors confers recessively inherited viral resistance in plants.. For example, loss of Essential for poteXvirus Accumulation 1 (EXA1) in Arabidopsis thaliana confers resistance to potexviruses.

    Plasmid Preparation:

    Article Title: Interaction of EXA1 and eIF4E Family Members Facilitates Potexvirus Infection in Arabidopsis thaliana.
    Article Snippet: Plant viruses depend on a number of host factors for successful infection.. Deficiency of critical host factors confers recessively inherited viral resistance in plants.. For example, loss of Essential for poteXvirus Accumulation 1 (EXA1) in Arabidopsis thaliana confers resistance to potexviruses.

    Article Title: Genome-wide analysis of the Tritipyrum WRKY gene family and the response of TtWRKY256 in salt-tolerance
    Article Snippet: .. Following the manufacturer’s instructions, the amplified fragment was digested with Sac / Spe I and BamH I/ Kpn I and then inserted into the pBI121 vector using T4-DNA ligase (Takara). ..

    Article Title: Genetic transformation of cotton with a harpin-encoding gene hpa Xoo confers an enhanced defense response against different pathogens through a priming mechanism
    Article Snippet: .. The BamH1- and Sac1-digested hpa1 Xoo fragment was ligated into a pBI121 vector (Clontech, Palo Alto, CA, USA) to generate the recombinant binary vector pBI35S- hpa1 Xoo -nptII, which contained a neomycin phosphotransferase II (nptII) with a nopaline synthase (nos) promoter and terminator, a CaMV35S promoter, an hpa1 Xoo insert, and a nopaline synthase terminator (Figure ). ..

    Article Title: Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings
    Article Snippet: The coxIV::EYFP was subsequently removed from this vector using the BamHI and SacI sites that were included in primers LM128 and LM131, respectively. .. The coxIV-EYFP BamHI/SacI fragment was then inserted into pBI121 (Clontech) at BamHI/SacI, thereby replacing the β-glucuronidase gene of the pBI121 vector (Clontech), forming pBI121-CoxIV::EYFP. ..

    Article Title: Unstable anthocyanin pigmentation in Streptocarpus sect. Saintpaulia (African violet) is due to transcriptional selectivity of a single MYB gene.
    Article Snippet: The VectorBuilder ID of each vector is listed: SiMYB1 ID: VB210520-1064sfu, SiMYB2-Long ID: VB210520-1068fhv, SibHLH1 ID: VB210520-1053zmv. .. The pBI121 vector was digested with BamHI (Takara) and SacI (Takara) to remove the GUS coding region. ..

    Polymerase Chain Reaction:

    Article Title: Interaction of EXA1 and eIF4E Family Members Facilitates Potexvirus Infection in Arabidopsis thaliana.
    Article Snippet: Plant viruses depend on a number of host factors for successful infection.. Deficiency of critical host factors confers recessively inherited viral resistance in plants.. For example, loss of Essential for poteXvirus Accumulation 1 (EXA1) in Arabidopsis thaliana confers resistance to potexviruses.

    Amplification:

    Article Title: Interaction of EXA1 and eIF4E Family Members Facilitates Potexvirus Infection in Arabidopsis thaliana.
    Article Snippet: Plant viruses depend on a number of host factors for successful infection.. Deficiency of critical host factors confers recessively inherited viral resistance in plants.. For example, loss of Essential for poteXvirus Accumulation 1 (EXA1) in Arabidopsis thaliana confers resistance to potexviruses.

    Article Title: Genome-wide analysis of the Tritipyrum WRKY gene family and the response of TtWRKY256 in salt-tolerance
    Article Snippet: .. Following the manufacturer’s instructions, the amplified fragment was digested with Sac / Spe I and BamH I/ Kpn I and then inserted into the pBI121 vector using T4-DNA ligase (Takara). ..

    Article Title: Suppression of DS1 Phosphatidic Acid Phosphatase Confirms Resistance to Ralstonia solanacearum in Nicotiana benthamiana
    Article Snippet: .. The full-length open reading frame of DS1 was amplified with the primers DS1ox-S and DS1ox-A using pGEMDS1 as the template and cloned into pGEMT-Easy, yielding pGEMDS1-2. pGEMDS1-2 was digested with BamHI and Sac I (Takara Bio), and ligated into the pBI121 vector (Clontech, Tokyo, Japan) digested with the same enzymes. ..

    Virus:

    Article Title: Increasing the production of limonin 17-β-D-glucopyranoside in hairy roots of Citrus × paradisi. cv Duncan by overexpression of limonoid UDP-glucosyltransferase via Agrobacterium rhizogenes mediated transformation
    Article Snippet: Limonin 17-β-D-glucopyranoside (LG) is a terpenoid compound with a high medicinal value produced in different Citrus species.. This substance has high anti-cancer and antioxidant properties and protects the liver from damage.. Although LG is tasteless, many compounds related to it are very bitter, although these bitter substances also have significant medicinal properties.

    Recombinant:

    Article Title: Genetic transformation of cotton with a harpin-encoding gene hpa Xoo confers an enhanced defense response against different pathogens through a priming mechanism
    Article Snippet: .. The BamH1- and Sac1-digested hpa1 Xoo fragment was ligated into a pBI121 vector (Clontech, Palo Alto, CA, USA) to generate the recombinant binary vector pBI35S- hpa1 Xoo -nptII, which contained a neomycin phosphotransferase II (nptII) with a nopaline synthase (nos) promoter and terminator, a CaMV35S promoter, an hpa1 Xoo insert, and a nopaline synthase terminator (Figure ). ..

    Clone Assay:

    Article Title: Suppression of DS1 Phosphatidic Acid Phosphatase Confirms Resistance to Ralstonia solanacearum in Nicotiana benthamiana
    Article Snippet: .. The full-length open reading frame of DS1 was amplified with the primers DS1ox-S and DS1ox-A using pGEMDS1 as the template and cloned into pGEMT-Easy, yielding pGEMDS1-2. pGEMDS1-2 was digested with BamHI and Sac I (Takara Bio), and ligated into the pBI121 vector (Clontech, Tokyo, Japan) digested with the same enzymes. ..



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